Development of an Electrochemical Immunosensor for Detection of Cardiac Troponin I at the Point-of-Care

Figures

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  Figure 1. Establishment of immunosensor for cTnI detection in whole blood samples. (A) Detection procedure used to obtain standard curves. (B) Investigation of consistency of individual anti-mouse cTnI antibody modified biochips after functionalization. (C) Investigation of consistency of individual anti-human cTnI antibody modified biochips after functionalization. (D) Standard curve of mouse cTnI (analysis was performed in triplicate). (E) Standard curve of human cTnI (analysis was performed in triplicate).

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  Figure 2. Establishment of cardiac damage mouse model. (A) Schedule of ISO injection in Balb/c mice. Mice were randomized into Group A (0.5 mL saline), Group B (100 mg/kg ISO in saline), and Group C (200 mg/kg ISO in saline). (B) IHC staining of heart tissue from mice in different groups at 100× and 400× magnification. Areas that were magnified to produce 400× images are boxed in red. (C) Statistical analysis of differences in cTnI measurements obtained using immunosensor between mice from the three groups. ** p ≤ 0.01, *** p ≤ 0.001. (D) Correlation between immunosensor detection method and standard clinical laboratory detection method in mouse model samples.

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  Figure 3. Comparison of immunosensor detection method to standard clinical laboratory detection method in human blood samples.