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Real-time action: The conversion of adenosine 5′-monophosphate (AMP) into adenosine by alkaline phosphatase was monitored in real time by exploiting a signaling DNA aptamer as a reporter. The aptamer has a higher affinity for adenosine than for AMP and releases readily distinguishable fluorescence signals upon complexation with AMP and after conversion of AMP into adenosine (see figure).
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Nutiu et al. (2004) studied this question.
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