What question did this study set out to answer?

Develop an iterative selection and amplification process to isolate high-affinity nucleic acid ligands for a target protein from randomized sequence pools.

August 3, 1990Science

Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

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Authors

CTCraig TuerkMorehead State University LGLarry GoldSomaLogic (United States)

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Implication

In vitro selection experiment demonstrates exponential enrichment of high-affinity RNA ligands targeting T4 DNA polymerase, suggesting broad utility for generating target-specific nucleic acid...

Key Points

Develop an iterative selection and amplification process to isolate high-affinity nucleic acid ligands for a target protein from randomized sequence pools.
Alternated iterative rounds of target-bound ligand selection with enzymatic amplification of the bound RNA species.
Randomized an eight-base region of an RNA that binds bacteriophage T4 DNA polymerase, creating a pool of 65,536 sequence variants.
Isolated two distinct RNA ligands from the 65,536-sequence pool: the wild-type bacteriophage sequence and a variant with four nucleotide substitutions.
Determined that the binding constants of the wild-type and quadruple-mutant RNA ligands to T4 DNA polymerase are equivalent.

Cite This Study

Tuerk et al. (1990) studied this question.

synapsesocial.com/papers/69d7dd2bf39344339dd18b41 https://doi.org/10.1126/science.2200121

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Implication

In vitro selection experiment demonstrates exponential enrichment of high-affinity RNA ligands targeting T4 DNA polymerase, suggesting broad utility for generating target-specific nucleic acid...

Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

Discussion

Member takes

Implication

Key Points

Cite This Study

Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

Discussion

Member takes

Implication

Key Points

Cite This Study

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