What question did this study set out to answer?

This research investigates how macromolecular crowding impacts the activity of T4 RNA ligase, specifically its ability to ligate oligo- and polynucleotides.

January 1, 1984Nucleic Acids ResearchOpen Access

Polymer-stimulated ligation: enhanced ligation of oligo- and polynucleotides by T4 RNA ligase in polymer solutions

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Authors

BHBarbara HarrisonNorth East Surrey College of Technology SZSteven B. ZimmermanUnited States Department of Health and Human Services

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Randomized trial examines enhanced ligation by T4 RNA ligase in polymer solutions, suggesting improvements for nucleic acid synthesis.

Key Points

This research investigates how macromolecular crowding impacts the activity of T4 RNA ligase, specifically its ability to ligate oligo- and polynucleotides.
Tested T4 RNA ligase activity in the presence of various concentrations of polyethylene glycol and other polymers.
Assessed the cyclization rate of oligoriboadenylates and the formation of large linear products.
Compared results with prior studies on DNA ligases under similar conditions.
Cyclization of oligoriboadenylates was enhanced up to 10-fold with high concentrations of polyethylene glycol.
Higher PEG concentrations facilitated the novel formation of large linear products from oligoriboadenylates.
Rate of T4 RNA ligase joining p(dT)10 to oligoriboadenylates increased significantly when PEG 8000 was present.

Cite This Study

Harrison et al. (1984) studied this question.

synapsesocial.com/papers/6a0eedbdaa1655e5fb2304a4 https://doi.org/10.1093/nar/12.21.8235

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Polymer-stimulated ligation: enhanced ligation of oligo- and polynucleotides by T4 RNA ligase in polymer solutions

Discussion

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Implication

Key Points

Cite This Study

Polymer-stimulated ligation: enhanced ligation of oligo- and polynucleotides by T4 RNA ligase in polymer solutions

Discussion

Member takes

Implication

Key Points

Cite This Study

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