Rare Recombinant GI.5[P4] Norovirus That Caused a Large Foodborne Outbreak of Gastroenteritis in a Hotel in Spain in 2021

Figures

• 

  FIG 1. Epidemiological curve of cases associated with the GI.5[P4] norovirus foodborne outbreak by date of illness onset. Probable cases with a known illness onset date were included (n = 110). For confirmed cases (n = 17), we used the illness onset date, except for the asymptomatic food handlers (n = 8), for which the sample collection date was used. Arrows indicate important events in the outbreak investigation. Subsequent genetic analysis of the GI.5P[4] strains identified them as members of subclusters I and II.

• 

  FIG 2. Phylogenetic analysis of the norovirus genomes identified in this study based on a 250-bp partial 3′-end sequence of ORF1 (A), a 274-bp partial 5′-end sequence of ORF2 (B), a complete ORF1 sequence (C), and complete ORF2 sequence (D). The sequences of closely related types (GI.4[P4] and GI.5[P5]) included in the analysis were those with highest scores when using the genomic regions ORF1 and ORF2 as queries for BLASTn (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Nucleotide sequences were analyzed using MEGA 5.0 software (http://megasoftware.net/) with the neighbor-joining method. Bootstrap values greater than 80% are shown. The robustness of the nodes was tested using 1,000 bootstrap replications. Scale bars indicate the number of nucleotide substitutions per site. Noncollapsed trees with taxon names can be found in Fig. S2A to D in the supplemental material.