Novel Picobirnaviruses in Respiratory and Alimentary Tracts of Cattle and Monkeys with Large Intra- and Inter-Host Diversity

Figures

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  Figure 1. Phylogenetic analysis of a partial RdRp gene of genogroup I and II picobirnaviruses (PBVs) discovered in the present study. The trees were constructed using the neighbor-joining method. Bootstrap values below 70% are not shown. The scale bar indicates the number of nucleotide substitutions per site. All the accession numbers are given as cited in GenBank. (a) One hundred and ninety-one nucleotide positions were included in the analysis and rooted with genogroup II human strains, shown in italics. All PBV strains detected in the same host species were highlighted in the same color, with the highlights in bold representing the PBVs discovered in respiratory specimens. If more than one sequence type was found in the same sample, each sequence type was numbered in the order of identification (e.g., Monkey/11T-1 and Monkey/11T-2 indicated that there were two sequence types found in the same monkey sample 11T). (b) One hundred and ninety-two nucleotide positions were included in the analysis and rooted with genogroup I human PBV strains. The genogroup II PBV characterized in this study is shown in bold.

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  Figure 2. Phylogenetic analysis of RdRp proteins of cattle and monkey picobirnaviruses (PBVs). The trees were constructed using the neighbor-joining method. Four hundred and seventy-five amino acid positions were included in the analysis. The scale bar indicates 0.05 substitutions per site. Bootstrap values below 70% are not shown. All PBV strains detected in the same host were highlighted with the solid dots/circles in the same color, with the highlights in bold representing the PBVs discovered in respiratory specimens. If more than one sequence type was found in the same sample, each sequence type was numbered in the order of identification (e.g., monkey/11T-1 and monkey/11T-2 indicated that there were two sequence types found in the same monkey sample 11T). The clade of each monkey PBV and their sampling locations (EN1: Lion Rock Site 1 and KS2: Kam Shan Site 2) are also indicated. All the accession numbers are given as cited in GenBank. GI and GII represent genogroup I and genogroup II PBVs, respectively.