Key result
Surface morphology of chick myogenic cells changes in association with the cell cycle, fusion, and subsequent development of myotubes, with microvilli and blebs appearing during late G2, mitosis, and fusion.
The study demonstrates that the surface morphology of chick myogenic cells changes dynamically during the cell cycle and myotube formation.
Preclinical chick cell findings are hypothesis-generating; leaves open relevance to mammalian myogenesis or cardiac muscle repair.
The surface morphology of chick myogenic cells during development in cell culture was examined by scanning electron microscopy. Myoblasts at the G1 and S phases of the cell cycle had a relatively smooth surface. In late G2 and mitosis, they had many microvilli and some blebs on their surfaces. Ca2+‐deficient fusion‐arrested myoblasts had a relatively smooth surface. When the cells underwent cell fusion, many microvilli, small spherical protrusions, and some blebs appeared on their surface. In newly formed myotubes, the surface over the nucleus was smooth whereas that over perinuclear regions had many flat excrescences and other surface protrusions. This mosaic appearance of the surface was less prominent in striated myotubes. Scanning electron microscopy combined with fluorescence microscopy using rhodamine‐labeled erabutoxin b revealed that sites of accumulation of acetylcholine receptor had a smooth surface. These results suggest that changes in surface structure occur in association with the cell cycle, fusion and subsequent development of myotubes.
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Masuko et al. (1983) studied Chick myogenic cells in culture. Cell cycle progression and cell fusion was evaluated on Surface morphology changes. Surface morphology of chick myogenic cells changes in association with the cell cycle, fusion, and subsequent development of myotubes, with microvilli and blebs appearing during late G2, mitosis, and fusion.
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