Key result
The peptide circPPP1R12A-73aa, translated from circPPP1R12A, promoted the proliferation of non-small cell lung cancer cells by activating the AKT signaling pathway.
Why the study?
Recent literature indicated cancer cell malignancy is modulated by circPPP1R12A via translated protein, but its role and latent mechanisms in NSCLC remained to be investigated.
CircPPP1R12A encodes a 73-amino acid peptide that promotes non-small cell lung cancer proliferation via the AKT signaling pathway.
Should not change NSCLC practice; hypothesis-generating for circPPP1R12A-73aa in animal models and requires human validation.
BACKGROUND: Recent literature have indicated that the malignancy of cancer cells is modulated by hsa_circ_0000423 (named circPPP1R12A) through the way of translating protein. Herein, we investigated the role and latent mechanisms of circPPP1R12A in Non-Small Cell Lung Cancer (NSCLC). METHODS: CircPPP1R12A expression was measured by qRT-PCR. The malignancy of NSCLC was determined by CCK-8, TUNEL assay, Wound healing, Transwell and Western blotting assays. The underlying mechanisms of circPPP1R12A were confirmed by Western blotting and qRT-PCR assays. RESULTS: CircPPP1R12A expression in NSCLC tissues was higher than that of neighboring normal tissues. CircPPP1R12A showed an upregulated expression in NSCLC cells. Upregulation of circPPP1R12A could promote the cell viability of NSCLC cells and reduce the apoptosis of NSCLC cells, while it could not promote cell invasion and migration. The reduction of cell viability and apoptosis was discovered in NSCLC cells with the silencing of circPPP1R12A, but circPPP1R12A knockdown does not inhibit cell invasion and migration. There was something interesting that circPPP1R12A encoding protein circPPP1R12A-73aa was found in NSCLC cells. Mutations in circPPP1R12a-73AA might disrupt the function of circPPP1ra-73AA in A549 and H1299 cells. Next, we found that circPPP1R12A caused the increased growth of NSCLC cells by activating AKT signaling pathway. CONCLUSION: In summary, our study proved that NSCLC cell proliferation was promoted by circPPP1R12A-73aa translated from circPPP1R12A through the AKT pathway, which could throw some light on the understanding of the mechanism of NSCLC.
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Zhao et al. (2022) studied Non-Small Cell Lung Cancer (NSCLC) (n=20). circPPP1R12A and its encoded peptide circPPP1R12A-73aa vs. Control vectors, si-control, or adjacent normal tissues was evaluated on Cell proliferation and AKT signaling pathway activation. The peptide circPPP1R12A-73aa, translated from circPPP1R12A, promoted the proliferation of non-small cell lung cancer cells by activating the AKT signaling pathway.
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