Key result
A novel TaqMan probe-based dual real-time PCR assay demonstrated high sensitivity and specificity, detecting NADC34-like PRRSV in 27 of 251 clinical samples.
Why the study?
The NADC34-like strain of PRRSV has become more prevalent across pig farms in China, but a reliable diagnostic method for clinical detection of this strain has been absent.
The developed TaqMan dual real-time PCR assay provides an accurate and sensitive tool for detecting NADC34-like PRRSV in clinical diagnostics and outbreak management in pig farms.
May improve targeted PRRSV surveillance in Chinese swine herds; leaves open prospective field validation before routine adoption.
Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus that induces reproductive disorders in sows and respiratory diseases in growing pigs. Recently, the NADC34-like strain of PRRSV has become more prevalent, with outbreaks occurring across pig farms in China. However, a reliable diagnostic method for the clinical detection of this strain has been absent. This study developed a TaqMan probe-based dual real-time quantitative PCR assay targeting the M and GP5 genes to specifically identify the NADC34-like PRRSV strain. The assay exhibited high specificity, detecting exclusively the NADC34-like strain without cross-reactivity with other PRRSV strains. The detection limits for pMD-M and pMD-GP5 plasmids were 2.67 × 10² and 1.35 × 10¹ copies/μL, respectively, indicating high assay sensitivity. The assay also demonstrated excellent reproducibility, with coefficient of variation (CV) values for both recombinant plasmids below 2 %. Among 251 clinical samples, 27 tested positive for NADC34-like PRRSV. This study establishes an accurate, sensitive, and reliable TaqMan dual real-time PCR assay for detecting NADC34-like PRRSV, offering a valuable tool for clinical diagnostics and outbreak management in pig farms.
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Han et al. (2025) studied NADC34-like PRRSV infection (n=251). TaqMan probe-based dual real-time PCR assay was evaluated on Detection of NADC34-like PRRSV. A novel TaqMan probe-based dual real-time PCR assay demonstrated high sensitivity and specificity, detecting NADC34-like PRRSV in 27 of 251 clinical samples.
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