Summary The polymerase chain reaction (PCR) method was reliably used for detection of potato leafroll virus (PLRV) RNA in dormant tubers from field‐grown plants and in vitro‐propagated microtubers. A simple RNA extraction from tuber and microtuber tissue allowed reverse transcription and PCR amplification of viral sequences. It was also demonstrated that ELISA could be used reliably for detection of PLRV in crude sap extracts of dormant microtubers produced in vitro but not in tubers from field‐grown plants.
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Spiegel et al. (1993) studied this question.
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