Key result
Examination of spiral defects in motility assays provides a simple measure of the elementary forces exerted by motor proteins such as myosin and kinesin.
Spiral defects in motility assays can be used as a simple method to estimate the elementary forces of motor proteins such as myosin and kinesin.
May simplify motor protein force assays in research; leaves open validation for cardiac mechanics applications.
In a commonly used motility assay, cytoskeletal filaments are observed as they glide over a surface coated with motor proteins. Defects in the motion frequently interrupt the flow of filaments. Examination of one such defect, in which a filament adopts a spiral form and rotates about a fixed point, provides a simple measure of the force exerted by the motor proteins. We demonstrate the universality of this approach by estimating the elementary forces of both myosin and kinesin.
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Bourdieu et al. (1995) studied this question. Motility assay spiral defects analysis was evaluated on Motor protein force estimation. Examination of spiral defects in motility assays provides a simple measure of the elementary forces exerted by motor proteins such as myosin and kinesin.
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