Anti-Obesity Effects of Black Barley Ferment Extract in 3T3-L1 Adipocytes and Obese Mice Induced by High Fat Diet

This study was undertaken to verify the anti-obesity effects of black barley ferment extract (BBFE) in 3T3-L1 adipocytes and obese mice.The anti-obesity effects of black barley extract (BBE) and BBFE were investigated by measuring Oil Red O staining in 3T3-L1 adipocytes.The staining results revealed that compared to BBE, exposure to BBFE significantly reduced lipid accumulation in cells.Compared to the untreated high-fat (HF) diet group, oral administration of BBFE (100, 300, and 500 mg/kg, body weight/daily for 8 weeks) in HF diet-induced obese mice resulted in reduced body weight, adipose tissue weight, food efficiency ratio, and serum levels of triglyceride, leptin, and insulin.Quantitative real-time PCR results determined that the elevated mRNA expression levels of adipogenesis-related genes of obese mice were significantly suppressed by BBFE.These results indicate the potential efficacy of BBFE in the prevention of obesity-related diseases.