What question did this study set out to answer?

To identify and map the matrix metalloproteinase binding site on the N-terminal domain of tissue inhibitor of metalloproteinases-2 (N-TIMP-2).

November 1, 1997Biochemistry

Mapping the Binding Site for Matrix Metalloproteinase on the N-Terminal Domain of the Tissue Inhibitor of Metalloproteinases-2 by NMR Chemical Shift Perturbation

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Authors

RWRichard A. WilliamsonAllegheny Health Network MCMark D. CarrUniversity of Leicester TFT.A. FrenkielThe Francis Crick Institute

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Implication

Biophysical NMR study reveals the matrix metalloproteinase binding site on N-TIMP-2, highlighting an interaction surface that overlaps with the conserved OB-fold topology.

Key Points

To identify and map the matrix metalloproteinase binding site on the N-terminal domain of tissue inhibitor of metalloproteinases-2 (N-TIMP-2).
Formed a stable complex between N-TIMP-2 and the catalytic domain of stromelysin-1 (N-MMP-3).
Measured backbone amide (1H and 15N) NMR chemical shift perturbations upon complex formation to identify contacting residues.
Significant amide chemical shift changes mapped to a broad surface on one face of N-TIMP-2, localized to residue clusters 1–11, 27–41, 68–73, 87–90, and 97–104.
The mapped MMP interaction interface overlaps with the OB-fold binding surface observed in homologous five-stranded beta-barrel proteins.

Cite This Study

Williamson et al. (1997) studied this question.

synapsesocial.com/papers/6a9ce1e296e7f648e9a8afba https://doi.org/10.1021/bi9712091

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Implication

Biophysical NMR study reveals the matrix metalloproteinase binding site on N-TIMP-2, highlighting an interaction surface that overlaps with the conserved OB-fold topology.

Mapping the Binding Site for Matrix Metalloproteinase on the N-Terminal Domain of the Tissue Inhibitor of Metalloproteinases-2 by NMR Chemical Shift Perturbation

Discussion

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Implication

Key Points

Cite This Study

Mapping the Binding Site for Matrix Metalloproteinase on the N-Terminal Domain of the Tissue Inhibitor of Metalloproteinases-2 by NMR Chemical Shift Perturbation

Discussion

Member takes

Implication

Key Points

Cite This Study

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