Actin‐binding protein Anillin promotes the progression of gastric cancer in vitro and in mice

Figures

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  FIGURE 1. ANLN is high expression in human GC tissues. A, ANLN level in GC tissues and normal tissues analyzed from bioinformatic analysis. (For tumor tissues and normal tissues, n = 408 and 211 respectively, from TCGA dataset). B, ANLN expression levels were shown in most types of cancers. ANLN expression level in GC tissue samples (C) and normal tissues (D) identified by IHC assays (100× and 200× magnification, respectively)

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  FIGURE 2. ANLN is downregulated in MGC‐803 and SGC‐7901 cells after ANLN shRNA transfection. Quantitative PCR analysis A, and immunoblot images B, verified downregulation of ANLN in MGC‐803 and SGC‐7901 cells transfected with ANLN shRNA plasmids. *P < .05

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  FIGURE 3. ANLN depletion promotes reduction of GC cell proliferation, migration, and invasion. A and B) Colony formation (A) assays and CCK‐8 assays (B) were performed to evaluate the proliferative ability of MGC‐803 and SGC‐7901 cell transfected with negative control (NC) or ANLN shRNA plasmids. C and D, Scratch assays (C) and transwell assays (D) were conducted to evaluate cell migration and cell invasion after ANLN knockdown. *P < .05

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  FIGURE 4. ANLN depletion inhibits tumor formation in vivo. A, Tumor growth curve of control or ANLN stably depletion MGC‐803 cells were injected into nude mice, and tumor volume was evaluated each week (n = 6) (left). Representative images of isolated tumor from each group. B, ANLN expression level in excised xenograft tumor tissues was analyzed by IHC analysis. *P < .05 and **P < .01