Key result
Midazolam and diazepam directly depressed cardiac function by decreasing the Ca2+ transient and beating rate in a concentration-dependent manner, an effect prevented by Bay K8644.
Midazolam and diazepam act as direct cardiac depressants by decreasing Ca2+ transients and beating rate via L-type Ca2+ channels in foetal mouse myocytes.
May caution benzodiazepine use in perinatal care; leaves open translation from foetal mouse myocytes to human neonates.
We have investigated the effects of midazolam and diazepam on intracellular calcium (Ca2+) handling in foetal mouse ventricular myocytes using the Ca2+-sensitive fluorescent indicator, indo-1. We also investigated separately whether flumazenil or the L-type Ca2+ channel agonist, Bay K8644, antagonized these myocardial depressive effects. Midazolam and diazepam decreased the Ca2+ transient and beating rate in a concentration-dependent manner, and these decreases were prevented by Bay K8644. Flumazenil did not antagonize the myocardial depressive effects. In myocytes whose sarcoplasmic reticulum was inhibited by ryanodine, midazolam and diazepam had the same potent cardiodepressive effects. Midazolam and diazepam are direct cardiac depressants, which decrease the Ca2+ transient and beating rate, and the L-type Ca2+ channel is important in the negative inotropism and chronotropism caused by these drugs.
No takes yet. Share an insight, caveat, or question.
Nonaka et al. (1997) studied this question. Midazolam and diazepam was evaluated on Ca2+ transient and beating rate. Midazolam and diazepam directly depressed cardiac function by decreasing the Ca2+ transient and beating rate in a concentration-dependent manner, an effect prevented by Bay K8644.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: