Besides some usual fatty acids, the seed oil ofSabastiana brasiliensis (Euphorbiaceae) contains up to 39% (estimated by ultraviolet spectroscopy) of α‐parinaric acid (cis, trans, trans, cis‐9, 11, 13, 15‐octadecatetraenoic acid). The fatty acids were analyzed by gas chromatography and gas chromatography/mass spectrometry as their methyl esters. The structure of α‐parinaric acid was proven by a combination of chemical and spectroscopic methods, conducted with the crude oil, the methyl ester mixture, and the isolated fatty acid methyl ester. Complete assignment of the1H and13C nuclear magnetic resonance (NMR) shifts of α‐parinaric acid was carried out by two‐dimensional NMR experiments
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Spitzer et al. (1996) studied this question.
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