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New polyhydroxylated cardiac steroids (I, II, III, IV, V and VI) were found in nuchal gland secretions of the snake, Rhabdophis tigrinus. The steroids contained an α-pyron ring at position 17 as those in bufodienolides but were not conjugated with suberoyl arginine. Hydroxylation occurred at position 3, 5, 11, 14; 3, 7, 11, 14, or 3, 5, 14, 16, respectively. This is the first report that the snake (Rhabdophis tigrinus) contains cardiac steroids in the nuchal glands.
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Akizawa et al. (1985) studied this question.
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