Key result
Direct sequencing and phylogenetic analysis of the S1 gene hypervariable regions successfully typed 7 field isolates of infectious bronchitis virus that were untypable by RT-PCR and RFLP.
Population
7 untyped field isolates of infectious bronchitis virus obtained in 1999 and 2000, and reference strains of…
Comparison
Direct sequencing followed by phylogenetic… vs Reverse transcriptase-polymerase chain reaction…
Design
Preclinical
Authors
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May aid typing of untypable IBV isolates; leaves open routine adoption without further validation.
Sequencing and phylogenetic analysis of the HVR 1 and HVR 2 regions of the S1 gene provides an effective method for typing infectious bronchitis virus isolates that cannot be typed by standard RT-PCR and RFLP.
Lee et al. (2003) studied Infectious bronchitis virus (IBV) (n=7). Direct sequencing and phylogenetic analysis of HVR 1 and HVR 2 in the S1 gene vs. RT-PCR and RFLP was evaluated on Typing of field isolates. Direct sequencing and phylogenetic analysis of the S1 gene hypervariable regions successfully typed 7 field isolates of infectious bronchitis virus that were untypable by RT-PCR and RFLP.
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