Purification and Properties of DPNH Peroxidase in Lactobacillus Casei

1.From the cells of a strain of Lactobacillus casei a flavin nucleotide-linked DPNH peroxidase was isolated in an almost homogeneous state, and it was revealed to have flavin adenine dinucleotide as prosthetic group. The preparation possessed weak activities of DPNH oxidase and dehydrogenase.2. The enzyme itself showed absorption maxima at 370 and 446mμ, and in the presence of DPNH there appeared a broad absorption at around 550mμ conconitantly with partial disappearance of the absorption at around 450mμ. The absorption at around 450mμ. The absorption at around 550 mμ disappeared on addition of hydrogen peroxide together with DPNH to the enzyme.