What question did this study set out to answer?

Determine the sequence conservation of the gluD gene and the expression of glutamate dehydrogenase across diverse Clostridium difficile ribotypes.

February 2, 2012Journal of Clinical MicrobiologyOpen Access

Glutamate Dehydrogenase Is Highly Conserved among Clostridium difficile Ribotypes

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Determine the sequence conservation of the gluD gene and the expression of glutamate dehydrogenase across diverse Clostridium difficile ribotypes.
Assayed 77 distinct Clostridium difficile ribotypes in vitro, including epidemic strains ARL 002, ARL 027, and ARL 106.
Evaluated gluD gene conservation, glutamate dehydrogenase (GDH) protein expression, and reactivity in standard GDH detection assays.
The gluD gene was highly conserved and GDH was consistently expressed in vitro across all 77 tested C. difficile ribotypes.
All 77 ribotypes, including ARL 002, ARL 027, and ARL 106, showed positive reactivity in assays designed to detect C. difficile GDH.

Authors

RCRobert J. CarmanMacquarie University KWK. N. WickhamTechLab (United States)LCL. ChenTechLab (United States)

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Implication

Laboratory analysis reveals universal glutamate dehydrogenase conservation across 77 Clostridium difficile ribotypes, highlighting its utility as a reliable diagnostic biomarker.

Cite This Study

Carman et al. (2012) studied this question.

synapsesocial.com/papers/6a9d674ed8a02169be46e023 https://doi.org/10.1128/jcm.05600-11

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Implication

Laboratory analysis reveals universal glutamate dehydrogenase conservation across 77 Clostridium difficile ribotypes, highlighting its utility as a reliable diagnostic biomarker.

Glutamate Dehydrogenase Is Highly Conserved among Clostridium difficile Ribotypes

Key Points

Discussion

Implication

Cite This Study

Glutamate Dehydrogenase Is Highly Conserved among Clostridium difficile Ribotypes

Key Points

Discussion

Implication

Cite This Study

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