Key result
Classic Human Astrovirus was detected in 10.3% of clinical samples from pediatric hospitalized patients with acute gastroenteritis in Uruguay, with HAstV-1 being the most common genotype.
Cross-Sectional (n=175)
Classic human astrovirus is a relevant etiologic agent in severe cases of pediatric acute gastroenteritis in Uruguay, with a prevalence of 10.3% among hospitalized children.
HAstV merits consideration in testing for severe pediatric gastroenteritis in Uruguay; leaves open need for regional surveillance and prospective studies.
Classic Human Astrovirus (Classic HAstV) are one of the most important causes of pediatric acute gastroenteritis (AGE), after rotaviruses and arguably caliciviruses. The aim of this study was to determine the molecular epidemiology of Classic HAstV from 175 clinical samples, being 153 stools and 22 vomits, collected from pediatric hospitalized patients with AGE in Salto city, Uruguay, from January 2011 to December 2012. Classic HAstV were detected and genotyped by using a qualitative Retro Transcription-Polimerase Chain Reaction (RT-PCR) directed to the Open Reading Frame-2 (ORF2) region C. Amplicons were sequenced and phylogenetic analyses were carried out in order to determine genotypes and lineages. Classic HAstV were detected in 18 out of 175 analyzed samples (10.3%) and 14 of them (78.0%) were successfully sequenced being 6 (42.8%) classified as HAstV-1 (1a lineage), 4 (28.6%) as HAstV-2 (2c lineage), and 4 (28.6%) as HAstV-3 (3c lineage). A higher detection of Classic HAstV infections was observed in autumn for both years of surveillance, and the majority of the positive cases were observed in 2011. The group of children between 2 and 5 years old presented the higher percentage of infections. To our knowledge, the present study represents the first report of astrovirus from acute gastroenteritis cases in Uruguay, evidencing its role as a relevant etiologic agent in severe cases of this disease.
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Lopez et al. (2017) conducted a cross-sectional in Acute gastroenteritis (n=175). Classic Human Astrovirus (Classic HAstV) infection was evaluated on Detection of Classic HAstV. Classic Human Astrovirus was detected in 10.3% of clinical samples from pediatric hospitalized patients with acute gastroenteritis in Uruguay, with HAstV-1 being the most common genotype.
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