Key result
Human astrovirus strain Yuc8 associates with extracellular vesicles, facilitating cell infection without trypsin activation and protecting virions from neutralizing antibodies.
Why the study?
Human astroviruses cause viral gastroenteritis and have been reported in extraintestinal organs, but it is not clear how the virus reaches these sites.
Human astrovirus associates with extracellular vesicles, which enhances infectivity and protects the virus from neutralizing antibodies, suggesting a novel pathway for viral dissemination.
May indicate EV-mediated astrovirus spread evading antibodies; hypothesis-generating for human pathogenesis and therapies.
Viral gastroenteritis has a global distribution and represents a high risk for vulnerable population and children under 5 years because of acute diarrhea, fever and dehydration. Human astroviruses (HAstV) have been identified as the third most important cause of viral gastroenteritis in pediatric and immunocompromised patients. Furthermore, HAstV has been reported in biopsies taken from patients with encephalitis, meningitis and acute respiratory infection, yet it is not clear how the virus reaches these organs. In this work we tested the possibility that the released astrovirus particles could be associated with extracellular vesicles. Comparison between vesicles purified from astrovirus- and mock-infected cells showed that infection with HAstV Yuc8 enhances production of vesicles larger than 150 nm. These vesicles contain CD63 and Alix, two markers of vesicular structures. Some of the extracellular virus was found associated with vesicular membranes, and this association facilitates cell infection in the absence of trypsin activation and protects virions from neutralizing antibodies. Our findings suggest a new pathway for HAstV spread and might represent an explanation for the extraintestinal presence of some astrovirus strains. Importance Astroviruses are an important cause of diarrhea in children; recently some reports have found these viruses in extra-intestinal organs, including the central nervous system, causing unexpected clinical disease. In this work we found that human astrovirus strain Yuc8 associates with extracellular vesicles, possibly during or after their cell egress. The association with vesicles seems to increase astrovirus infectivity in less susceptible cells, and renders virus particles insensitive to neutralization by antibodies. These data suggest that extracellular vesicles could represent a novel pathway for astrovirus to disseminate outside the gastrointestinal tract.
No takes yet. Share an insight, caveat, or question.
Carlos et al. (2021) studied Human astrovirus infection. Human astrovirus strain Yuc8 infection vs. Mock infection was evaluated on Viral infectivity and association with extracellular vesicles. Human astrovirus strain Yuc8 associates with extracellular vesicles, facilitating cell infection without trypsin activation and protecting virions from neutralizing antibodies.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: