What question did this study set out to answer?

To determine the antifungal efficacy of chitosan against Alternaria alternata f. sp. lycopersici by measuring its impact on fungal growth, toxin synthesis, and toxin virulence.

June 1, 1997HortScienceOpen Access

Effect of Chitosan on Growth and Toxin Production by Alternaria alternata f. sp. lycopersici

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Authors

MRM.V. Bhaskara Reddy EBEssaïd Ait Barka FCF. Castaigne

Discussion

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Overview

In vitro study demonstrates that chitosan suppresses mycelial growth and attenuates toxin virulence in Alternaria alternata, highlighting its potential as a biopolymer antifungal agent.

Key Points

To determine the antifungal efficacy of chitosan against Alternaria alternata f. sp. lycopersici by measuring its impact on fungal growth, toxin synthesis, and toxin virulence.
Cultured Alternaria alternata in potato dextrose broth with chitosan concentrations ranging from 100 to 6,400 μg·ml⁻¹ across a 15-day incubation period.
Assessed fungal growth, morphology, spore viability, protein leakage, and minimum inhibitory concentrations for both mycelial growth and toxin production.
Evaluated culture filtrate virulence on tomato discs by measuring tissue phospholipid degradation, electrolyte leakage, xylanase activity, and pectin methylesterase activity.
High chitosan concentrations significantly inhibited both fungal growth and toxin production, while lower concentrations suppressed toxin synthesis more markedly than mycelial growth.
Chitosan exposure induced hyphal contortion, cellular aggregation, abnormal branching, cellular protein leakage, and reduced spore viability despite stimulating sporulation.
Culture filtrates from chitosan-treated samples showed reduced virulence on tomato discs, causing less electrolyte leakage, lower phospholipid degradation, and suppressed activation of xylanase and pectin methylesterase.

Cite This Study

Reddy et al. (1997) studied this question.

synapsesocial.com/papers/6a9d7bb230bdd6b10046e49c https://doi.org/10.21273/hortsci.32.3.467f

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June 1, 1997HortScienceOpen Access

Effect of Chitosan on Growth and Toxin Production by Alternaria alternata f. sp. lycopersici

View Full Paper

Ask AI

Bookmark

Authors

MRM.V. Bhaskara Reddy EBEssaïd Ait Barka FCF. Castaigne

Discussion

Member takes

Overview

In vitro study demonstrates that chitosan suppresses mycelial growth and attenuates toxin virulence in Alternaria alternata, highlighting its potential as a biopolymer antifungal agent.

Key Points

To determine the antifungal efficacy of chitosan against Alternaria alternata f. sp. lycopersici by measuring its impact on fungal growth, toxin synthesis, and toxin virulence.
Cultured Alternaria alternata in potato dextrose broth with chitosan concentrations ranging from 100 to 6,400 μg·ml⁻¹ across a 15-day incubation period.
Assessed fungal growth, morphology, spore viability, protein leakage, and minimum inhibitory concentrations for both mycelial growth and toxin production.
Evaluated culture filtrate virulence on tomato discs by measuring tissue phospholipid degradation, electrolyte leakage, xylanase activity, and pectin methylesterase activity.
High chitosan concentrations significantly inhibited both fungal growth and toxin production, while lower concentrations suppressed toxin synthesis more markedly than mycelial growth.
Chitosan exposure induced hyphal contortion, cellular aggregation, abnormal branching, cellular protein leakage, and reduced spore viability despite stimulating sporulation.
Culture filtrates from chitosan-treated samples showed reduced virulence on tomato discs, causing less electrolyte leakage, lower phospholipid degradation, and suppressed activation of xylanase and pectin methylesterase.

Cite This Study

Reddy et al. (1997) studied this question.

synapsesocial.com/papers/6a9d7bb230bdd6b10046e49c https://doi.org/10.21273/hortsci.32.3.467f

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