Key result
A newly developed HPLC method successfully quantified individual long-chain fatty acyl-CoA esters in pig and rat heart and skeletal muscle.
Population
Freeze-clamped tissue of pig and rat hearts and rat skeletal muscle
Comparison
High performance liquid chromatography on a… vs A separate procedure that measured total CoA by…
Design
Preclinical
Authors
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Facilitates acyl-CoA metabolic studies in animal heart and muscle; leaves open human validation and clinical translation.
The described HPLC method allows for the quantitation of individual long-chain fatty acyl-CoA species in heart and skeletal muscle tissue, providing a tool to study molecular species under various metabolic conditions.
MOLAPARASTSALESS et al. (1988) studied this question. HPLC method for determination of long-chain fatty acyl-CoA esters vs. Separate procedure measuring total CoA by HPLC after alkaline hydrolysis was evaluated on Total long-chain acyl-CoA esters. A newly developed HPLC method successfully quantified individual long-chain fatty acyl-CoA esters in pig and rat heart and skeletal muscle.