Key result
The combination of homogenization and heat treatment with an in-house real-time PCR demonstrated 100% sensitivity for adenovirus detection, outperforming traditional virus culture which had a sensitivity of 69.2%.
Why the study?
Does an in-house real-time PCR following homogenization and heat treatment provide accurate and cost-effective detection of adenovirus compared to commercial extraction and viral culture in clinical swabs?
Observational (n=196)
No
Does an in-house real-time PCR following homogenization and heat treatment provide accurate and cost-effective detection of adenovirus compared to commercial extraction and viral culture in clinical swabs?
Homogenization and heat treatment combined with an in-house real-time PCR provides a highly sensitive and cost-effective alternative to commercial nucleic acid extraction and viral culture for adenovirus detection.
May enable more sensitive adenovirus detection in swabs; leaves open prospective validation before routine use.
Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an "in-house" real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method showed performance characteristics equivalent to viral DNA obtained from a commercial nucleic acid extraction. In addition, the in-house real-time PCR outperformed traditional testing strategies using virus culture, with sensitivities of 100% and 69.2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture.
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Al-Siyabi et al. (2013) conducted an observational in Suspected adenovirus infection (respiratory or conjunctivitis) (n=196). In-house real-time PCR following homogenization and heat treatment vs. Virus culture was evaluated on Sensitivity for adenovirus detection. The combination of homogenization and heat treatment with an in-house real-time PCR demonstrated 100% sensitivity for adenovirus detection, outperforming traditional virus culture which had a sensitivity of 69.2%.
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