Key result
A nested PCR method for detecting adenoviruses and enteroviruses in environmental water samples demonstrated a sensitivity of 1 to 10 viral particles and yielded more positive samples than tissue culture.
Population
Sewage and polluted river samples
Comparison
Virus concentration by centrifugation, elution… vs Tissue culture analysis
Design
Other
Authors
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May improve virus detection in water monitoring; leaves open standardization before routine clinical or regulatory adoption.
A nested PCR method following specific concentration and extraction steps is highly sensitive for detecting adenoviruses and enteroviruses in environmental water samples.
Gironés et al. (1995) studied this question. Nested PCR amplification vs. Tissue culture analysis was evaluated on Detection of adenovirus and enterovirus. A nested PCR method for detecting adenoviruses and enteroviruses in environmental water samples demonstrated a sensitivity of 1 to 10 viral particles and yielded more positive samples than tissue culture.
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