April 1, 1995Applied and Environmental MicrobiologyOpen Access

Reverse transcription PCR to detect enteroviruses in surface water

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Population

Water samples

Comparison

Reverse transcription PCR of the highly… vs Enterobacterial contamination determined by PCR…

Design

Other

Authors

MGM. GilgenUniversity of Bern BWBernhard WegmüllerFoederatio Medicorum Helveticorum PBPaul W BurkhalterUniversity of Bern

Discussion

Member takes

Implication

RT-PCR assay detects enteroviruses in surface water; supports E. coli correlation but leaves open routine monitoring adoption.

Structured PICO

Population

Water samples (tap water artificially spiked with poliovirus vaccine, and n=40 surface water samples from public beaches by rivers and lakes)

Intervention

Reverse transcription PCR of the highly conserved noncoding region at the 5' end of virus RNA combined with Southern hybridization, following two-step filtration and RNA isolation via magnetic beads

Comparator

Enterobacterial contamination determined by PCR and classical microbiological methods (Escherichia coli)

Outcome

Detection of enteroviruses

A newly developed RT-PCR procedure allows for simple, fast, and efficient detection of enteroviruses in large water samples for routine water quality monitoring.

Cite This Study

Gilgen et al. (1995) studied this question.

synapsesocial.com/papers/6a8bcc8ebc51310b2bdec87f https://doi.org/10.1128/aem.61.4.1226-1231.1995

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Reverse transcription PCR to detect enteroviruses in surface water

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Reverse transcription PCR to detect enteroviruses in surface water

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider