Rapid Detection of Enteroviruses in Small Volumes of Natural Waters by Real-Time Quantitative Reverse Transcriptase PCR
Population
Seawater and freshwater samples from watersheds in Los Angeles, California
Authors
Discussion
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Member takes
Implication
HA filters optimize enterovirus recovery in freshwater; leaves open best practices for seawater monitoring.
Structured PICO
P
Population
Seawater and freshwater samples from watersheds in Los Angeles, California
I
Intervention
Filtration using negatively charged filters (cellulose acetate/nitrate or glass filter) followed by real-time quantitative reverse transcriptase PCR (qRT-PCR)
O
Outcome
Viral recovery rate and detection of enteroviruses
A rapid filtration-qRT-PCR approach can detect enteroviruses in environmental water samples in under 8 hours, offering a promising method for monitoring viral contamination.
Cite This Study
Fuhrman et al. (2005) studied this question.