Key result
An optimized RT-PCR method with universal primers successfully detected enteroviruses in water samples with a detection limit of 38CCID50.
An optimized RT-PCR method with universal primers was established for the rapid detection of enteroviruses in water samples with a detection limit of 38 CCID50.
A method for rapid detection of enteroviruses in water sample was established by using RT-PCR with universal primers with PV1, PV2, PV3 and CVB3 as the reference viruses. The conditions for simultaneous detection of different enteroviruses were experimentally determined and the detection limit of virus was found to be 38CCID50. Virus concentration, RNA extraction and RT-PCR operation were the main factors affecting the sensitivity of virus detection. By applying the procedures to water samples with seeded PV1 viruses, the interference of impurities in practical waters on enteroviruses detection was investigated. The method was proved to be suitable for practical application.
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Zhang et al. (2009) studied Enterovirus detection in water. RT-PCR with universal primers was evaluated on Detection limit of enteroviruses. An optimized RT-PCR method with universal primers successfully detected enteroviruses in water samples with a detection limit of 38CCID50.
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