Key result
Organic solvent extraction recovers ~11-fold more adenovirus than silica methods from sewage sludge.
Why the study?
The study aimed to compare two nucleic acid extraction methods for recovering enteric viruses from activated sludge to determine the more effective technique.
Population
Activated sludge samples inoculated with AdV, HAV, PV, and RV
Comparison
Organic solvent-based extraction method vs silica extraction method
Design
Comparative laboratory study
Authors
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May aid environmental virus surveillance; leaves open translation to routine monitoring or diagnostics.
An organic solvent-based extraction method is more effective than a silica-based method for recovering enteric viruses from sewage sludge.
Schlindwein et al. (2009) studied Enteric virus recovery from sewage sludge. Organic solvent-based extraction method vs. Silica extraction method was evaluated on Recovery of enteric viruses from seeded samples. An organic solvent-based extraction method recovered more adenovirus (20% vs 1.8%), poliovirus, and hepatitis A virus than a silica method from seeded sewage sludge samples.
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