Key result
Techniques using 10% beef extract at pH 9 with sonication or 0.3 M NaCl-7% beef extract at pH 7.5 with Freon yielded the highest viral titers for simultaneous detection of enteroviruses in sludge.
Population
16 different liquid and solid urban sludge samples
Comparison
8 different virus extraction techniques vs Comparison among the 8 techniques
Design
Other
Authors
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Optimizes enterovirus recovery from sludge for surveillance; extends prior methods but leaves open validation before routine adoption.
p-value: p=0.011
Two specific extraction techniques using beef extract solutions are optimal for simultaneous detection of infectious enterovirus particles and genomes in urban sludge.
Monpoeho et al. (2001) studied Enterovirus contamination in urban sludge (n=16). Viral elution techniques (Techniques 5 and 6) vs. Other elution techniques was evaluated on Efficiency of viral extraction (enterovirus genomes by RT-PCR and infectious particles by cell culture) (p=0.011). Techniques using 10% beef extract at pH 9 with sonication or 0.3 M NaCl-7% beef extract at pH 7.5 with Freon yielded the highest viral titers for simultaneous detection of enteroviruses in sludge.
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