What question did this study set out to answer?

To evaluate and compare the engraftment and metastatic growth of RhoC-expressing human melanoma cells across four different immunodeficient mouse strains.

May 15, 2009Clinical Cancer ResearchOpen Access

Immunodeficient Mouse Strains Display Marked Variability in Growth of Human Melanoma Lung Metastases

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Authors

BCBeatriz M. CarrenoCalifornia University of Pennsylvania JGJoel R. GarbowWashington University in St. Louis GKGrant R. KolarMayo Clinic

Discussion

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Implication

Comparative animal study reveals variable melanoma metastasis across immunodeficient mouse strains, indicating natural killer cell absence is required for reliable human tumor engraftment.

Key Points

To evaluate and compare the engraftment and metastatic growth of RhoC-expressing human melanoma cells across four different immunodeficient mouse strains.
Monitored serial metastatic growth of RhoC-expressing human A375 melanoma cells in scid, NOD-scid, NOD-scid beta2m(null), and NOD-scid IL2Rgamma(null) mice using bioluminescence, MRI, and histopathology.
Assessed murine natural killer (NK) cell cytotoxicity against human melanoma cell lines and primary cells using chromium-51 release assays and antibody blockade of the murine NKG2D receptor.
Metastatic melanoma growth was delayed and variable in scid and NOD-scid mice, whereas NOD-scid beta2m(null) and NOD-scid IL2Rgamma(null) strains showed rapid engraftment.
Active NK cells in scid, NOD-scid, and NOD-scid beta2m(null) mice killed melanoma cells via murine NKG2D recognition of human MICA/B ligands, while NOD-scid IL2Rgamma(null) mice exhibited absolute NK cell deficiency.

Cite This Study

Carreno et al. (2009) studied this question.

synapsesocial.com/papers/6a9d90de9f8b6241c867fbea https://doi.org/10.1158/1078-0432.ccr-08-2502

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Implication

Comparative animal study reveals variable melanoma metastasis across immunodeficient mouse strains, indicating natural killer cell absence is required for reliable human tumor engraftment.

Immunodeficient Mouse Strains Display Marked Variability in Growth of Human Melanoma Lung Metastases

Discussion

Member takes

Implication

Key Points

Cite This Study

Immunodeficient Mouse Strains Display Marked Variability in Growth of Human Melanoma Lung Metastases

Discussion

Member takes

Implication

Key Points

Cite This Study

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