Key result
A 71-nucleotide sequence containing a consensus slippery sequence (GGGUUUA) and a 3' pseudoknot is essential for -1 ribosomal frameshifting in the Saccharomyces cerevisiae double-stranded RNA virus.
The study demonstrates that a pseudoknot structure is essential for -1 ribosomal frameshifting in the S. cerevisiae double-stranded RNA virus.
No immediate clinical implications in yeast model; leaves open conservation in human viral pathogens.
The large double-stranded RNA of the Saccharomyces cerevisiae (yeast) virus has two large overlapping open reading frames on the plus strand, one of which is translated via a -1 ribosomal frameshift. Sequences including the overlapping region, placed in novel contexts, can direct ribosomes to make a -1 frameshift in wheat germ extract, Escherichia coli and S. cerevisiae. This sequence includes a consensus slippery sequence, GGGUUUA, and has the potential to form a pseudoknot 3' to the putative frameshift site. Based on deletion analysis, a region of 71 nucleotides including the potential pseudoknot and the putative slippery sequence is sufficient for frameshifting. Site-directed mutagenesis demonstrates that the pseudoknot is essential for frameshifting.
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Tzeng et al. (1992) studied Saccharomyces cerevisiae double-stranded RNA virus. Ribosomal frameshift sequence (71 nt) with pseudoknot vs. Mutated sequences or reverse orientation was evaluated on Frameshifting efficiency. A 71-nucleotide sequence containing a consensus slippery sequence (GGGUUUA) and a 3' pseudoknot is essential for -1 ribosomal frameshifting in the Saccharomyces cerevisiae double-stranded RNA virus.
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