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Procedures for the large scale isolation of pure triglycerides and fractions rich in nontriglyceride components from Span rapeseed oil are described. Fractionation of Brassica campestris var. Span rapeseed oil by molecular distillation yielded 4 triglyceride fractions, all of which contained traces of sterol esters. An additional triglyceride fraction rich in free and esterified sterols and other volatile components was obtained from the oil. Separation by adsorption chromatography of Span rapeseed oil yielded three fractions; A) a pure triglyceride ffaction; B) a triglyceride fraction rich in sterol esters; and C) another fraction containing free sterols and other polar components.
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Kramer et al. (1975) studied this question.
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