A new method for ultra-fast flow injection analysis-mass spectrometry (FIA-MS) has been developed. An eight probe autosampler system implemented for FIA-MS analysis of combinatorial libraries synthesized in microtiter plates has enabled sample analysis times to be dramatically reduced as compared to microtiter plate analyses using single arm autosamplers. Compounds contained within the microtiter plate wells are processed eight at a time, eight samples in under a minute. Using this multiple probe autosampler, it has been possible to process a single microtiter plate in 12 minutes. To facilitate the data acquisition and processing of the samples, the sample data acquisition list, originally generated for registration of compounds synthesized in a microtiter plate format, was modified using an Excel macro into the format of an injection sample list for mass spectral data acquisition. Further, an automated data processing script was developed to process the multi-injection analysis results. Applications of the probe multiple autosampler to the analysis of combinatorial libraries synthesized in microtiter plates and compounds purified into deep well microtiter plates is presented.
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Wang et al. (1998) studied this question.