Key result
A novel whole-body clearing and staining methodology successfully visualized calcium deposits in situ across the entire mdx mouse model, revealing significant differences in deposit distribution among muscle groups (p < 0.001).
Why the study?
Does whole-body clearing and alizarin red S staining allow for rapid and high-resolution screening of calcium deposits in the mdx mouse model of Duchenne muscular dystrophy?
Does whole-body clearing and alizarin red S staining allow for rapid and high-resolution screening of calcium deposits in the mdx mouse model of Duchenne muscular dystrophy?
p-value: p=<0.001
A novel whole-body clearing and imaging methodology enables precise in situ localization and quantification of calcium deposits in the mdx mouse model of Duchenne muscular dystrophy.
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Novel clearing method enables whole-body calcium mapping in mdx mice; leaves open translation to human DMD screening.
Bożycki et al. (2018) studied Duchenne muscular dystrophy (animal model) (n=15). Whole-body clearing (CUBIC protocol) and alizarin red S staining vs. Control mice (no calcium deposits) was evaluated on Distribution and volume of calcified deposits in skeletal muscles (p=<0.001). A novel whole-body clearing and staining methodology successfully visualized calcium deposits in situ across the entire mdx mouse model, revealing significant differences in deposit distribution among muscle groups (p < 0.001).
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