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A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.
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Deutsch et al. (1996) studied this question.
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