What question did this study set out to answer?

To determine the role of protein ubiquitination and the ubiquitin-proteasome system in facilitating the replication and lifecycle of coxsackievirus B3.

July 9, 2008PLoS ONEOpen Access

Ubiquitination Is Required for Effective Replication of Coxsackievirus B3

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Authors

XSXiaoning SiBC Cancer Agency GGGuang GaoUniversity of British Columbia JWJerry WongNational Institutes of Health

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Implication

In vitro study reveals that ubiquitin-proteasome system hijacking promotes Coxsackievirus B3 replication in host cells, indicating a potential target for antiviral therapeutics.

Key Points

To determine the role of protein ubiquitination and the ubiquitin-proteasome system in facilitating the replication and lifecycle of coxsackievirus B3.
Assessed CVB3 RNA replication, protein synthesis, and viral titers in infected HeLa cells under proteasome inhibition using in situ hybridization, Western blotting, and plaque assays.
Knocked down ubiquitin expression using small-interfering RNA (siRNA) and analyzed post-translational modification of viral polymerase 3D via immunoprecipitation and deubiquitinating enzyme inhibitors.
Proteasome inhibition and siRNA-mediated ubiquitin knockdown substantially decreased CVB3 RNA replication, protein synthesis, and final viral titers.
CVB3 infection caused an accumulation of protein-ubiquitin conjugates and a reduction in free ubiquitin without altering baseline 20S proteasome enzymatic activity.
Coxsackievirus polymerase 3D was post-translationally modified by ubiquitination, indicating this modification is required for its function in viral genome transcription.

Cite This Study

Si et al. (2008) studied this question.

synapsesocial.com/papers/6a9db603843eea9644fd0341 https://doi.org/10.1371/journal.pone.0002585

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Implication

In vitro study reveals that ubiquitin-proteasome system hijacking promotes Coxsackievirus B3 replication in host cells, indicating a potential target for antiviral therapeutics.

Ubiquitination Is Required for Effective Replication of Coxsackievirus B3

Discussion

Member takes

Implication

Key Points

Cite This Study

Ubiquitination Is Required for Effective Replication of Coxsackievirus B3

Discussion

Member takes

Implication

Key Points

Cite This Study

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