Key result
Coxsackievirus A16 infection of 16HBE cells induced significant changes in the expression of 172 proteins, which are mainly involved in metabolic processes and immune- and neuro-associated pathways.
Why the study?
No vaccines or antiviral compounds exist for Coxsackievirus A16, and its pathogenic mechanisms and host-cell interactions remain poorly elucidated.
CV-A16 infection in 16HBE cells significantly alters the expression of 172 proteins involved in metabolic, immune, and neuroactive pathways, providing insights into its molecular pathogenesis.
Alters protein pathways in bronchial cells in vitro; leaves open in vivo relevance for CV-A16 pathogenesis.
Hand, foot and mouth disease (HFMD) caused by Coxsackievirus A16 (CV-A16) is a global health concern worldwide. There are no vaccines or antiviral compounds available to either prevent or treat CV-A16 infection which may trigger severe neurological complications, and even lead to death. Moreover, its pathogenic mechanisms and pathophysiology are still poorly elucidated. To increase our understanding of the interaction of CV-A16 with the host cell, we analyzed changes in the proteome of 16HBE cells in response to CV-A16 infection using tandem mass tag (TMT) in combination with LC-MS/MS. It was identified and quantified 6615 proteins and there were 172 proteins showed a significant alteration during CV-A16 infection. To validate the proteomics data, 3 randomly selected proteins exhibited consistent changes in protein expression with the TMT results using a Western blotting and immunofluorescence method. Then, functional enrichment analysis showed that these differentially expressed proteins mainly involved in various biological processes and signaling pathways, such as metabolic process, Cytokine-cytokine receptor interaction, B cell receptor signaling pathway, Neuroactive ligand-receptor interaction, etc. And further bioinformatics analysis revealed that these differentially expressed proteins contained distinct domains, localized in different subcellular components, and established a complex network. In conclusion, results from this study have helped elucidate the molecular pathogenesis of CV-A16 and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods.
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Hu et al. (2022) studied Coxsackievirus A16 infection. Coxsackievirus A16 infection vs. Mock-incubated cells was evaluated on Differentially expressed proteins. Coxsackievirus A16 infection of 16HBE cells induced significant changes in the expression of 172 proteins, which are mainly involved in metabolic processes and immune- and neuro-associated pathways.
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