Die Sterine und Bufadienolide der Haut von Bufo formosus B OULENGER . Über Krötengifte, 31. Mitteilung

In the skin of the Japanese toad Bufo formosus BOULENGER three sterols and 19 bufadienolides have been traced by thin layer and paper chromatography. Combining different preparative chromatographic methods a complete qualitative separation could be achieved. From 490 animals the following amounts of the 3 sterols and 16 crystalline bufadienolides were obtained (amounts in mgs): cholesterol (ca. 20000; only a fraction was purified), 7α‐hydroxy‐cholesterol (1.5, identification tentative), 7β‐hydroxy‐cholesterol (8.5), cinobufagin (40), bufalone (8.5), bufotalone (12), bufalin (353), 3‐epi‐bufalin (32), cinobufotalin (54), marinobufagin (45), bufotalin (1357), bufotalinin (13), telocinobufagin (42), desacetyl‐cinobufagin (18), desacetyl‐bufotalin (76), arenobufagin (143), hellebrigenin = bufotalidin (25), desacetyl‐cinobufotalin (18), gamabufotalin (2136). The following three were obtained in amorphous from: resibufogenin (74, identified as crystalline O‐acetyl‐derivative), argentinogenin (14) and U5 (7, not identified).