What is the clinical evidence from this study?

Study design: Other. Population: Healthy (isolated rat diaphragm). Intervention: Insulin vs. No insulin. Primary outcome: Synthesis of rapidly labeled RNA (incorporation of C14-adenine or P32).

November 1, 1963Proceedings of the National Academy of SciencesOpen Access

An Influence of Insulin on the Synthesis of a Rapidly Labeled Rna by Isolated Rat Diaphragm

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Key result

Insulin added in vitro to isolated rat diaphragm increased the synthesis of a rapidly labeled RNA fraction resembling messenger RNA and increased the specific activity of ribosomal RNA.

Authors

IWIra G. WoolUniversity of Chicago AMAlan MunroUniversity of Cambridge

Discussion

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Implication

May support insulin's transcriptional effects in muscle; leaves open relevance to human metabolism or therapy.

PICO

Population

Isolated hemidiaphragms from nonfasted male Wistar or Sprague-Dawley rats weighing 110-130 gm.

Intervention / Comparator

Insulin vs No insulin (0.1 unit/ml)

Primary Outcome

Synthesis of rapidly labeled RNA (incorporation of C14-adenine or P32)

Limitations

For mammalian cells there is no assurance that the RNA that is rapidly synthesized in the nucleus serves exactly the same role as it does in bacteria.

Cite This Study

Wool et al. (1963) studied Healthy (isolated rat diaphragm). Insulin vs. No insulin was evaluated on Synthesis of rapidly labeled RNA (incorporation of C14-adenine or P32). Insulin added in vitro to isolated rat diaphragm increased the synthesis of a rapidly labeled RNA fraction resembling messenger RNA and increased the specific activity of ribosomal RNA.

synapsesocial.com/papers/6a9dea9082302eba5bd9e39b https://doi.org/10.1073/pnas.50.5.918

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November 1, 1963Proceedings of the National Academy of SciencesOpen Access

An Influence of Insulin on the Synthesis of a Rapidly Labeled Rna by Isolated Rat Diaphragm

View Full Paper

Ask AI

Bookmark

Key result

Insulin added in vitro to isolated rat diaphragm increased the synthesis of a rapidly labeled RNA fraction resembling messenger RNA and increased the specific activity of ribosomal RNA.

Authors

IWIra G. WoolUniversity of Chicago AMAlan MunroUniversity of Cambridge

Discussion

Member takes

Implication

May support insulin's transcriptional effects in muscle; leaves open relevance to human metabolism or therapy.

PICO

Population

Isolated hemidiaphragms from nonfasted male Wistar or Sprague-Dawley rats weighing 110-130 gm.

Intervention / Comparator

Insulin vs No insulin (0.1 unit/ml)

Primary Outcome

Synthesis of rapidly labeled RNA (incorporation of C14-adenine or P32)

Limitations

For mammalian cells there is no assurance that the RNA that is rapidly synthesized in the nucleus serves exactly the same role as it does in bacteria.

Cite This Study

synapsesocial.com/papers/6a9dea9082302eba5bd9e39b https://doi.org/10.1073/pnas.50.5.918

Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

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