The free choline concentration in the rat brain was found to be 26.3 nanomoles per gram of brain tissue. This value was obtained through use of 6-second microwave irradiation for killing animals and inactivating enzymes, followed by a pyrolysis-gas chromatographic assay procedure. The identities of compounds measured from brain samples were verified by mass spectrometry.
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Stavinoha et al. (1974) studied this question.
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