Quantitative determination of heparin released lipoprotein lipase activity in human plasma

A method for quantitative determination of heparin released lipoprotein lipase (LPL) activity has been developed. The importance of standardization of substrate and albumin in the assay system has been investigated. Determination of glycerol release in the assay system has been shown to have advantages compared with titration of fatty acids released. Enzyme plasma samples frozen for four months decreased in activity. The method seemed to be specific for emulsified triglycerides and the LPL activity was 98% inhibited by 1 M NaCl. However, there was a lack of reproducibility of the substrate when different lots were used. Analytical error of the method was approximately 5% and there was good reproducibility.