Growth and Tissue Formation from Single, Isolated Tobacco Cells in Microculture

Single, isolated cells of tobacco divided and grew to form small colonies of over 50 cells in microcultures with a medium containing fresh liquid coconut milk, but in the absence of neighboring cells or nurse tissue. Subsequently, the mass of cells obtained from the single cell, when transferred to agar coconut milk medium, established itself as a clone of callus tissue. Some of these single-cell clones showed differentiation of many tracheid-like cells and shoots with small leaves.