Studies on antibiotics from the genus Bacillus. XII. The constituent amino acids and fatty acid of antibiotic 333-25.

Measurement of the optical rotational activities of the constituent amino acids of antibiotic 333-25 identified 2,4-diaminobutyric acid (D-form 1, L-form 4), L-leucine (2) and D-phenylalanine (1). The fatty acid constituent was determined to be beta-hydroxy anteisononanoic acid by gas chromatography, nuclear magnetic resonance and mass spectra. Differentiation from the structure of antibiotic EM 49 is discussed.