Key result
Lipoprotein lipase activator concentration in cow serum increased linearly with increasing dietary fat and was related to the concentration of total lipid in plasma.
A newly developed laboratory assay demonstrated that lipoprotein lipase activator concentration in cow serum increases linearly with dietary fat.
Caution against human application; extends bovine lipid metabolism knowledge, hypothesis-generating for translation.
A routine laboratory assay to evaluate relative concentrations of lipoprotein lipase activator (apo C-II) in cow serum was developed. The assay was linear for at least 120 min after an initial, unexplained, lag ime of 13 to 15 min. Half-maximal activation was in the range of 1 to 2% serum in the assay. Inhibition of activation was indicated at high amounts (10%) of serum. Activation from plasma was half that from serum, presumably caused by an increase in substrate Km in the presence of heparin. Use of glyceryl tri[9,10-3H] oleate yielded excessively high blanks; [2-3H] glyceryl triolein is suggested for routine assay. Relative amounts of activator were not different between dry and lactating cows fed "conventional" diets. Activator concentration increased linearly with increasing dietary fat and was related to concentration of total lipid in plasma. The assay may provide a useful adjunct in studies on lipoprotein metabolism.
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Super et al. (1976) studied this question. Varying amounts of dietary fat vs. Conventional diets was evaluated on Relative concentrations of lipoprotein lipase activator (apo C-II). Lipoprotein lipase activator concentration in cow serum increased linearly with increasing dietary fat and was related to the concentration of total lipid in plasma.
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