Answers are grounded in this paper and cite their sources.
In this work an improved micropropagation protocol for Pinus pinaster Ait. using isolated cotyledons is presented. The variables studied during bud induction were embryo preculture, type and concentration of cytokinin and influence of illumination culture conditions. Shoot development and proliferation was obtained by transfer to hormone-free medium with activated charcoal. This protocol also describes a procedure for successful continuous multiplication using axillary shoots and brachyblasts formed during shoot development. Based on 86 per cent rooting success rate, at least 70 plantlets can be expected per seed after 16 weeks.
No takes yet. Share an insight, caveat, or question.
Álvarez et al. (2009) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: