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Alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD+) have been incorporated within rigid graphite epoxy matrices to yield renewable (by polishing) alcohol sensors. The enzyme and cofactor retain their bioactivity on confinement in the graphite epoxy, with the oxidation current of the NADH formed serving as the response. Because of the intimate contact between the biocatalytic and sensing sites, the reagentless sensor produces steady‐state signals within 15–30s. The influence of various experimental variables is explored for optimum analytical performance. Common polishing procedures are used to regenerate fresh bioactive surfaces.
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Wang et al. (1992) studied this question.
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