Key result
A newly developed multiplex PCR assay simultaneously detected and differentiated porcine parvovirus types 1 through 7 in various field samples with a minimum detection limit of 3×10^3 viral copies.
Why the study?
Although the epidemiological impacts of newly recognized parvoviruses PPV2 through PPV7 on pigs are largely unknown, continuous surveillance is needed.
The developed multiplex PCR assay provides a rapid, sensitive, and specific diagnostic tool for the simultaneous detection and epidemiological surveillance of seven porcine parvovirus genotypes in various field samples.
Supports ongoing PPV2–7 surveillance in European herds; leaves open their clinical impact pending controlled studies.
Porcine parvoviruses (PPVs) are small, nonenveloped DNA viruses that are widespread in the global pig population. PPV type 1 (PPV1) is a major causative agent of reproductive failure and has been recognized since the 1960s. In recent decades, novel PPVs have been identified and designated as PPVs 2 through 7 (PPV2~PPV7). Although the epidemiological impacts of these newly recognized parvoviruses on pigs are largely unknown, continuous surveillance of these PPVs is needed. The aim of this study was to develop an improved and efficient detection tool for these PPVs and to assess the developed method with field samples. Using 7 sets of newly designed primers, a multiplex polymerase chain reaction (mPCR) protocol was developed for the simultaneous detection of the seven genotypes of PPV (PPV1~PPV7). The sensitivity of the mPCR assay was analyzed, and the detection limit was determined to be 3×103 viral copies. The assay was highly specific in detecting one or more of the viruses in various combinations in specimens. The mPCR method was evaluated with 80 serum samples, 40 lung or lymph node samples and 40 intestine or fecal samples. When applied to these samples, the mPCR method could detect the 7 viruses simultaneously, providing rapid results regarding infection and coinfection status. In conclusion, the developed mPCR assay can be utilized as an effective and accurate diagnostic tool for rapid differential detection and epidemiological surveillance of various PPVs in numerous types of field samples.
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Kim et al. (2021) studied Porcine parvovirus infection (n=160). Multiplex PCR (mPCR) method vs. Simplex PCR was evaluated on Simultaneous detection and differentiation of PPV1 through PPV7. A newly developed multiplex PCR assay simultaneously detected and differentiated porcine parvovirus types 1 through 7 in various field samples with a minimum detection limit of 3×10^3 viral copies.
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