Key result
Novel calcium probes based on troponin C variants demonstrated ratio changes up to 140% and K(d)s ranging from 470 nm to 29 microm, with improved subcellular targeting properties.
Population
HEK293 cells and primary hippocampal neurons
Design
Preclinical
Authors
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May advance preclinical calcium imaging; leaves open clinical translation in cardiovascular research.
Novel genetically encoded calcium indicators based on troponin C and GFP provide improved dynamic ranges and subcellular targeting for studying cellular calcium dynamics.
Heim et al. (2004) studied Cellular calcium dynamics. Calcium probes based on troponin C variants vs. Calmodulin-based probes was evaluated on Ratio changes and K(d)s. Novel calcium probes based on troponin C variants demonstrated ratio changes up to 140% and K(d)s ranging from 470 nm to 29 microm, with improved subcellular targeting properties.
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